|
| Status |
Public on Jun 03, 2014 |
| Title |
375_I_RF |
| Sample type |
RNA |
| |
|
| Source name |
Gonads at stage I of Ruditapes decussatus sampled in Ria Formosa
|
| Organism |
Ruditapes decussatus |
| Characteristics |
tissue: gonads
Stage: I
sample data and location: between July 2011 and July 2012 in Ria Formosa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Extract-all (Eurobio) procedure.
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent Low input One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 2 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Gonads RNA of Ruditapes decussatus
|
| Data processing |
Data processed with FeatureExtraction v10.7.3.1 and normalized by quantile normalization using R statistical software
|
| |
|
| Submission date |
Sep 24, 2013 |
| Last update date |
Jun 03, 2014 |
| Contact name |
Massimo Milan |
| E-mail(s) |
massimo.milan@unipd.it
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17766 |
| Series (1) |
| GSE51150 |
A microarrays-based analysis of gametogenesis in two Portuguese populations of the European clam Ruditapes decussatus |
|
