|
Status |
Public on Sep 26, 2013 |
Title |
whi3 deletion in YPD replicate2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
whi3 cells
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype: whi3 strain background: S288C growth medium: YEP+2% glucose
|
Growth protocol |
Cells were grown in YEP medium supplemented with different carbon sources as indicated at 30°C to log phase for harvest.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Yeast RiboPure Kit (CAT#AM1926) following manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Samples were labeled using Agilent Quick Amp Labeling Kit (CAT#5190-0444) following manufacturer’s instructions
|
|
|
Channel 2 |
Source name |
WHI3
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genotype: WHI3 strain background: S288C growth medium: YEP+2% glucose
|
Growth protocol |
Cells were grown in YEP medium supplemented with different carbon sources as indicated at 30°C to log phase for harvest.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Yeast RiboPure Kit (CAT#AM1926) following manufacturer's instructions.
|
Label |
Cy5
|
Label protocol |
Samples were labeled using Agilent Quick Amp Labeling Kit (CAT#5190-0444) following manufacturer’s instructions
|
|
|
|
Hybridization protocol |
Labeled samples were hybridized to the Agilent Yeast (V2) Gene Expression Microarray, 8x15K, using Agilent Gene Expression Hybridization Kits (CAT#5188-5242) following manufacturer’s instructions
|
Scan protocol |
Scanned on an Agilent DNA microarray scanner, Model#: G2505B. Images were quantified using Agilent Feature Extraction Software (version 9.5.3.1).
|
Description |
Biological replicate 2 of 2. log phase whi3 deletion in YEP+2% glucose
|
Data processing |
Agilent Feature Extraction Software (v 9.5.3.1) was used for background subtraction and LOWESS normalization. Saturated and outlier spots were removed. The log ratios were averaged for the same probe.
|
|
|
Submission date |
Sep 25, 2013 |
Last update date |
Sep 26, 2013 |
Contact name |
Ying Cai |
E-mail(s) |
cai.ying@stonybrook.edu
|
Organization name |
Stony Brook University
|
Department |
Molecular Genetics and Microbiology
|
Lab |
Bruce Futcher
|
Street address |
Life Sciences Building Rm 363
|
City |
Stony Brook |
State/province |
New York |
ZIP/Postal code |
11794 |
Country |
USA |
|
|
Platform ID |
GPL9825 |
Series (2) |
|