|
| Status |
Public on Sep 26, 2013 |
| Title |
whi3 deletion in YPE replicate1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
whi3 cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: whi3
strain background: S288C
growth medium: YEP+3% ethanol
|
| Growth protocol |
Cells were grown in YEP medium supplemented with different carbon sources as indicated at 30°C to log phase for harvest.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Yeast RiboPure Kit (CAT#AM1926) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled using Agilent Quick Amp Labeling Kit (CAT#5190-0444) following manufacturer’s instructions
|
| |
|
| Channel 2 |
| Source name |
WHI3
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: WHI3
strain background: S288C
growth medium: YEP+3% ethanol
|
| Growth protocol |
Cells were grown in YEP medium supplemented with different carbon sources as indicated at 30°C to log phase for harvest.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Yeast RiboPure Kit (CAT#AM1926) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Samples were labeled using Agilent Quick Amp Labeling Kit (CAT#5190-0444) following manufacturer’s instructions
|
| |
|
| |
| Hybridization protocol |
Labeled samples were hybridized to the Agilent Yeast (V2) Gene Expression Microarray, 8x15K, using Agilent Gene Expression Hybridization Kits (CAT#5188-5242) following manufacturer’s instructions
|
| Scan protocol |
Scanned on an Agilent DNA microarray scanner, Model#: G2505B. Images were quantified using Agilent Feature Extraction Software (version 9.5.3.1).
|
| Description |
Biological replicate 1 of 2. log phase whi3 deletion in YEP+3% ethanol
|
| Data processing |
Agilent Feature Extraction Software (v 9.5.3.1) was used for background subtraction and LOWESS normalization. Saturated and outlier spots were removed. The log ratios were averaged for the same probe.
|
| |
|
| Submission date |
Sep 25, 2013 |
| Last update date |
Sep 26, 2013 |
| Contact name |
Ying Cai |
| E-mail(s) |
cai.ying@stonybrook.edu
|
| Organization name |
Stony Brook University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Bruce Futcher
|
| Street address |
Life Sciences Building Rm 363
|
| City |
Stony Brook |
| State/province |
New York |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
|
| Platform ID |
GPL9825 |
| Series (2) |
|
