|
| Status |
Public on Dec 31, 2006 |
| Title |
Muscle_freeze_1d_rep464 |
| Sample type |
RNA |
| |
|
| Source name |
Muscle, freeze injury, 1 day post-injury, sample 464
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6J
Gender: female
Age: 2-3 months
Tissue: Tibialis anterior muscle
|
| Biomaterial provider |
Petia Simeonova (NIOSH)
|
| Treatment protocol |
Injured using 6-mm diameter steel probe cooled to temperature of dry ice; applied for 10 seconds
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Homogenize in TRIzol followed by purification with Qiagen RNeasy Mini kit
|
| Label |
Phycoerythrin
|
| Label protocol |
As per Affmetrix Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
As per Affmetrix Expression Analysis Technical Manual
|
| Scan protocol |
As per Affmetrix Expression Analysis Technical Manual
|
| Description |
Mouse sacrificed at 1-day post-injury and injured tibialis anterior muscle excised
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Jul 30, 2006 |
| Last update date |
Jul 31, 2006 |
| Contact name |
Petia P Simeonova |
| E-mail(s) |
psimeonova@cdc.gov
|
| Phone |
304-285-6156
|
| Organization name |
NIOSH, CDC
|
| Street address |
1095 Willowdale Rd.
|
| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5413 |
Comparison of gene expression for contraction- and freeze-induced skeletal muscle injuries |
|
