NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1240511 Query DataSets for GSM1240511
Status Public on Dec 01, 2013
Title H4-II-E-C3_untreated_rep4
Sample type RNA
 
Source name H4-II-E-C3 cells untreated
Organism Rattus norvegicus
Characteristics cell line: H4-II-E-C3
Treatment protocol Exposures of 24 hours in duration were initiated once flasks were confluent using the test chemicals CoCl2.
Growth protocol MH1C1 and H4-II-E-C3 cells (ATCC, Manassas, VA) were grown in Dulbecco's Modified Eagle's Medium (DMEM; Lonza, Walkersville, MD) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA) and 10 mL Glutamax (Invitrogen) in T75 flasks incubated at 37 °C with 5% carbon dioxide.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Samples were hybridized on the GeneChip Rat Genome 230 2.0 Array using Affymetrix instrumentation according to the manufacturer’s instructions.
Scan protocol Microarrays were scanned on an Affymetrix GeneChip Scanner following the manufacturer's instructions.
Data processing Microarray data was processed for background adjustment, normalization, and summarization using the Robust Multi-Array Averaging method (RMA) using Partek Genomic Suite (GS) software (Version 6.6, St. Louis, MO).
 
Submission date Sep 26, 2013
Last update date Dec 01, 2013
Contact name Matthew Permenter
Organization name US Army Center for Environmental Health Research
Street address 568 Doughten Dr
City Fort Detrick
State/province MD
ZIP/Postal code 21758
Country USA
 
Platform ID GPL1355
Series (1)
GSE51207 Exposure to Cobalt Causes Transcriptomic and Proteomic Changes in Two Rat Liver Derived Cell Lines

Data table header descriptions
ID_REF
VALUE log2-RMA

Data table
ID_REF VALUE
1367452_at 12.2225
1367453_at 10.8323
1367454_at 10.3688
1367455_at 11.8286
1367456_at 11.3288
1367457_at 10.5667
1367458_at 7.62317
1367459_at 11.9067
1367460_at 12.6624
1367461_at 11.343
1367462_at 8.68052
1367463_at 12.1325
1367464_at 10.3937
1367465_at 10.6007
1367466_at 10.1256
1367467_at 10.8664
1367468_at 9.39909
1367469_at 12.8248
1367470_at 10.6212
1367471_at 10.5284

Total number of rows: 31099

Table truncated, full table size 577 Kbytes.




Supplementary file Size Download File type/resource
GSM1240511_571-008-101.CEL.gz 2.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap