|
| Status |
Public on Oct 01, 2013 |
| Title |
DRG_VEH_4 |
| Sample type |
RNA |
| |
|
| Source name |
ipsilateral L5 rat DRG, day 14, veh treated
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: injured L5 DRG
|
| Treatment protocol |
the HDACi MS-275 was delivered i.t. via an osmotic pump (30nmol/ day)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen tissue was homogenized in 500 μL Trizol, separated into phases using chloroform and cleaned up with a Qiagen RNA microkit (74004; Qiagen, Manchester, UK) following manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Ambion Whole transcript Expression Kit (Invitrogen)
|
| |
|
| Hybridization protocol |
hybridized to Affymetrix Rat Gene Arrays (Rat Gene 1.0ST) on a GeneChip Fluidics Station 450.
|
| Scan protocol |
Affymetrix Gene chips scanner
|
| Description |
Gene expression data from rat L5 DRG after SNT
|
| Data processing |
R, usual quality controls, RMA normalisation, DRG & spinal cord tissue were run in separate batches and analysed separately
|
| |
|
| Submission date |
Sep 30, 2013 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Franziska Denk |
| Organization name |
King's College London
|
| Street address |
Guy's Campus
|
| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE51295 |
HDAC inhibitors attenuate the development of hypersensitivity in models of neuropathic pain [DRG tissue] |
| GSE51296 |
HDAC inhibitors attenuate the development of hypersensitivity in models of neuropathic pain |
|
