|
Status |
Public on Sep 29, 2016 |
Title |
spermatogonian stem cell rep3 |
Sample type |
RNA |
|
|
Source name |
spermatogonian stem cell
|
Organism |
Mus musculus |
Characteristics |
strain: CD1 age: 6 days tissue: testis cell type: spermatogonian stem cell
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from germ line stem cells was extracted using Trizol reagent according to the manufacturer’s instructions.RNA that passed the quality check were hybridized to Mouse WG-6 v2.0 (Illumina).
|
Label |
biotin
|
Label protocol |
cDNA were prepared with Ambion illumina TotalPrep RNA amplification kits and labeled according standard manufacturer’s instructions.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
SSC3 replicate 3
|
Data processing |
The data were background corrected and normalized using quantile normalization with lumi package in R
|
|
|
Submission date |
Sep 30, 2013 |
Last update date |
Sep 29, 2016 |
Contact name |
Ji Wu |
E-mail(s) |
jiwu@sjtu.edu.cn
|
Organization name |
Shanghai Jiao Tong University
|
Street address |
No.800, Dongchuan Road, Minhang District
|
City |
Shanghai |
ZIP/Postal code |
200240 |
Country |
China |
|
|
Platform ID |
GPL6887 |
Series (1) |
GSE51313 |
Comparision of gene expression profiles between female germline stem cells and spermatogonia stem cells |
|