|
| Status |
Public on Feb 13, 2014 |
| Title |
AML3 + IL-3 16h |
| Sample type |
RNA |
| |
|
| Source name |
Primary AML MNCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary AML MNCs
treatment: + IL-3 16h
disease: Acute myeloid leukaemia
cytogenetics: normal
|
| Treatment protocol |
Mononuclear AML cells thawed overnight and cultured in IMDM with 10% FBS were treated +/- 15ng/mL hIL-3 for 6 or 16h.
|
| Growth protocol |
Mononuclear AML cells were thawed and recovered overnight in IMDM with 20% FBS and DNase (50U/mL). Non-viable cells were subsequently removed using Lymphoprep (Axis-Shield PLC). AML cells were then cultured in IMDM with 10% FBS and stimulated with IL-3 as described below.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the miRNeasy protocol (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to biotinylated sense DNA as per the Affymetrix Genechip Whole Transcript (WT) Sense Target Labeling Assay Manual.
|
| |
|
| Hybridization protocol |
Genechips were hybridised overnight at 45 degrees C for 17 hours in an Affymetrix Hybridisation oven.
|
| Scan protocol |
Genechips were scanned using the Affymetrix GSC3000 scanner.
|
| Data processing |
Raw CEL files were normalised with RMA method using aroma.affymetrix.
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
Feb 13, 2014 |
| Contact name |
Chung H Kok |
| E-mail(s) |
chung.kok@sahmri.com
|
| Organization name |
South Australia Health and Medical Research Institute
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5000 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE51402 |
Gene expression patterns in response to IL-3 in human AML patient mononuclear cells |
|
