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Sample GSM1244806 Query DataSets for GSM1244806
Status Public on Feb 13, 2014
Title AML4 no IL-3 6h
Sample type RNA
 
Source name Primary AML MNCs
Organism Homo sapiens
Characteristics cell type: Primary AML MNCs
treatment: no IL-3 6h
disease: Acute myeloid leukaemia
cytogenetics: normal
Treatment protocol Mononuclear AML cells thawed overnight and cultured in IMDM with 10% FBS were treated +/- 15ng/mL hIL-3 for 6 or 16h.
Growth protocol Mononuclear AML cells were thawed and recovered overnight in IMDM with 20% FBS and DNase (50U/mL). Non-viable cells were subsequently removed using Lymphoprep (Axis-Shield PLC). AML cells were then cultured in IMDM with 10% FBS and stimulated with IL-3 as described below.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the miRNeasy protocol (Qiagen)
Label Biotin
Label protocol Total RNA was converted to biotinylated sense DNA as per the Affymetrix Genechip Whole Transcript (WT) Sense Target Labeling Assay Manual.
 
Hybridization protocol Genechips were hybridised overnight at 45 degrees C for 17 hours in an Affymetrix Hybridisation oven.
Scan protocol Genechips were scanned using the Affymetrix GSC3000 scanner.
Data processing Raw CEL files were normalised with RMA method using aroma.affymetrix.
 
Submission date Oct 18, 2013
Last update date Feb 13, 2014
Contact name Chung H Kok
E-mail(s) chung.kok@sahmri.com
Organization name South Australia Health and Medical Research Institute
Street address North Terrace
City Adelaide
State/province South Australia
ZIP/Postal code 5000
Country Australia
 
Platform ID GPL6244
Series (1)
GSE51402 Gene expression patterns in response to IL-3 in human AML patient mononuclear cells

Data table header descriptions
ID_REF
VALUE log2 RMA normalised signal intensity

Data table
ID_REF VALUE
7892501 5.882368691
7892502 7.821808717
7892503 4.477901519
7892504 9.714146767
7892505 5.02589907
7892506 5.97742056
7892507 6.741476142
7892508 8.69432377
7892509 12.15220065
7892510 5.869916284
7892511 6.851557671
7892512 8.07021301
7892513 6.056150808
7892514 11.39896988
7892515 11.0440582
7892516 6.244256539
7892517 8.897163468
7892518 4.259922954
7892519 6.134917277
7892520 10.64964157

Total number of rows: 33252

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1244806_TS_7.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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