|
| Status |
Public on Nov 17, 2014 |
| Title |
Flk1Cre;ETV2 CKO YS-I |
| Sample type |
RNA |
| |
|
| Source name |
E9.5 Yolk sac
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: Embryonic day 9.5
tissue: Yolk sac
|
| Treatment protocol |
ETV2 was inactivated in FLK1+ cells by a loxP-Cre recombination approach.
|
| Growth protocol |
Embryos at day 9.5 of gestation were prepared from a scheduled mating.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
9.5.Mut.YSD.I
|
| Data processing |
The data were normalised using the default quantile normalisation with R/bioconductor package lumi version 2.15.1
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
Nov 17, 2014 |
| Contact name |
Chunfa Jie |
| E-mail(s) |
cjie.jhmi@gmail.com
|
| Phone |
3124043831
|
| Organization name |
Des Moines University
|
| Street address |
3200 Grand Ave
|
| City |
Des Moines |
| State/province |
IA |
| ZIP/Postal code |
50266 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE51407 |
Genome-wide analysis of gene expression of Flk1Cre;ETV2 CKO mouse yolk sacs. |
|
