|
| Status |
Public on Aug 27, 2014 |
| Title |
Liver of S. aurata exposed to 6.8°C [T1_CE_a] |
| Sample type |
RNA |
| |
|
| Source name |
Liver of S. aurata exposed to 6.8°C
|
| Organism |
Sparus aurata |
| Characteristics |
weight/length: 120±16 g body weight; 21±1 cm total length
exposed to: 6.8 ± 0.3 °C (cold groups)
tissue: liver
|
| Treatment protocol |
Sea breams (120±16 g body weight; 21±1 cm total length) caught in Valle Bonello were kept in 5 m3 circular recycling tanks and acclimated over one month at 16 °C. Fish were then exposed to 6.8 ± 0.3 °C (cold groups). Water was firstly gradually cooled from 16 °C to 12 °C at a rate of 1°C day -1 and then reduced from 12 °C to 6.8 °C within 24 h. S.aurata were fasted during the trial.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted according to QiAgen RNeasy MiniKit protocol for tissue samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
T1_CE_a
|
| Data processing |
Data were processed with FeatureExtraction v9.5 and filtering/cyclic lowess normalization were performednormalized using R2.6.1 statistical software.
|
| |
|
| Submission date |
Oct 18, 2013 |
| Last update date |
Aug 28, 2014 |
| Contact name |
Massimo Milan |
| E-mail(s) |
massimo.milan@unipd.it
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6467 |
| Series (1) |
| GSE51442 |
Transcriptome analysis of gilthead sea bream Sparus aurata liver upon exposure to low temperature |
|
