|
| Status |
Public on Jan 03, 2014 |
| Title |
IL-10 20 ng/ml |
| Sample type |
RNA |
| |
|
| Source name |
monocyte-derived macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
culture condition: IL-10 20 ng/ml
|
| Treatment protocol |
At day 7 cells were treated for 24 hours with Dexamethasone 100nM, LPS 10 ng/ml, IL-10 20 ng/ml, IL-13 20 ng/ml, IL-4 20 ng/ml or IFNy 20 ng/ml
|
| Growth protocol |
Monocytes were isolated from buffy coats of healthy blood donors by Ficoll and percoll density gradientsand culture in X Vivo-10 supplemented with 1% Autologous serum for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Total RNA isolation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Austin, TX, U.S.A.) according to the manufacturer’s specifications with an input of 200 ng total RNA.
|
| |
|
| Hybridization protocol |
Hybridization and washing were performed according to the Illumina Manual “Direct Hybridization Assay Guide”
|
| Scan protocol |
Scanning was performed on the Illumina iScan (Illumina, Inc., San Diego, CA, U.S.A.).
|
| Description |
1% autologous serum macrophages
|
| Data processing |
Image analysis and extraction of raw expression data was performed with Illumina GenomeStudio v2011.1 Gene Expression software with default settings (no background substraction and no normalization)
|
| |
|
| Submission date |
Oct 20, 2013 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
| GSE51446 |
Similarities and differences between human activated macrophages II |
|
