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Sample GSM1246695 Query DataSets for GSM1246695
Status Public on May 19, 2015
Title group 1-mutant-1
Sample type RNA
 
Source name H157 Squamous Cell Lung Cancer Cell Line (SCC)
Organism Homo sapiens
Characteristics cell line: H157
vector: tet repressor vector and a vector with a mutant form of PARD3 gene, NM_001184785.1 without exon 2
Treatment protocol Induction with doxocyclyn (1 ng/ul)
Growth protocol Cells where growth on RPMI media with 10% FBS, 1% PS
Extracted molecule total RNA
Extraction protocol Total mRNA where extrated using Trizol Reagent.
Label Cy3
Label protocol Amount of nucleic acid labeled: 100ng. Commercial One-Color Microarray-Based Gene Expression Analysis” v.6.6 kit by following manufacturer instructions. Agilent manual G4140-90040 of September 2012. Amplification: by RNA polymerases.
 
Hybridization protocol Microarray: SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent microarray design ID 039494)
Scan protocol Scanned on an G2505C DNA microarray scanner (Agilent).
Data processing Images were quantified using Agilent Feature Extraction Software (ver. 10.7). Date were processed using the RMA algorithm.
 
Submission date Oct 21, 2013
Last update date May 19, 2015
Contact name Manel Esteller
Organization name IDIBELL
Department PEBC
Lab Cancer Epigenetics
Street address Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
City L'Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platform ID GPL16699
Series (1)
GSE51506 Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form.

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
1 15.93
2 6.38
3 6.66
4 12.64
5 8.4
6 7.88
7 6.62
8 9.76
9 18.42
10 12.12
11 10.74
12 8.5
13 9.29
14 6.59
15 6.32
16 6.59
17 10.72
18 10.29
19 10.47
20 11.44

Total number of rows: 62976

Table truncated, full table size 668 Kbytes.




Supplementary file Size Download File type/resource
GSM1246695_1D21.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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