|
Status |
Public on May 19, 2015 |
Title |
group 1-mutant-1 |
Sample type |
RNA |
|
|
Source name |
H157 Squamous Cell Lung Cancer Cell Line (SCC)
|
Organism |
Homo sapiens |
Characteristics |
cell line: H157 vector: tet repressor vector and a vector with a mutant form of PARD3 gene, NM_001184785.1 without exon 2
|
Treatment protocol |
Induction with doxocyclyn (1 ng/ul)
|
Growth protocol |
Cells where growth on RPMI media with 10% FBS, 1% PS
|
Extracted molecule |
total RNA |
Extraction protocol |
Total mRNA where extrated using Trizol Reagent.
|
Label |
Cy3
|
Label protocol |
Amount of nucleic acid labeled: 100ng. Commercial One-Color Microarray-Based Gene Expression Analysis” v.6.6 kit by following manufacturer instructions. Agilent manual G4140-90040 of September 2012. Amplification: by RNA polymerases.
|
|
|
Hybridization protocol |
Microarray: SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent microarray design ID 039494)
|
Scan protocol |
Scanned on an G2505C DNA microarray scanner (Agilent).
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (ver. 10.7). Date were processed using the RMA algorithm.
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|
|
Submission date |
Oct 21, 2013 |
Last update date |
May 19, 2015 |
Contact name |
Manel Esteller |
Organization name |
IDIBELL
|
Department |
PEBC
|
Lab |
Cancer Epigenetics
|
Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
City |
L'Hospitalet de Llobregat |
State/province |
Barcelona |
ZIP/Postal code |
08908 |
Country |
Spain |
|
|
Platform ID |
GPL16699 |
Series (1) |
GSE51506 |
Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form. |
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