|
Status |
Public on Oct 23, 2013 |
Title |
Smoke_testis rep 3 |
Sample type |
RNA |
|
|
Source name |
Adult testis from cigarette smoke exposed mouse
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: adult exposed to: cigarette smoke in utero tissue: testis
|
Treatment protocol |
C57BL/6 5 week-old female mice were exposed via the nose-only to cigarette smoke [twelve 3R4F reference cigarettes (University of Kentucky, USA) twice/day, five times per week, for 12-18 weeks]. Each exposure lasted 60 minutes. Control mice received room air. In total, 27 mice underwent cigarette smoke exposure. Eleven week-old female mice exposed to cigarette smoke for 6 weeks were separated into groups of three and housed with a single control stud male aged 7-8 weeks with proven fertility for a maximum of 12 weeks. Females were monitored every second day for post-coital plugs and pregnancy. Pregnant females were separated into single cages and litter sizes/pup weights recorded. Smoke exposure via dams continued throughout mating/pregnancy/lactation until weaning of pups at 21days post birth.
|
Growth protocol |
All experimental procedures involving the use of animals were performed with the approval of the University of Newcastle’s Animal Care and Ethics Committee (ACEC). Specific pathogen-free adult female (6-week-old) C57BL/6 mice were obtained from the animal services unit and maintained according to the recommendations prescribed by the ACEC. Mice were housed under a controlled lighting regime (16L:8D) at 21–22°C and supplied with food and water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA (approximately 3 µg) was isolated from adult ovaries using a QIAGEN RNeasy Mini Kit (Hilden, Germany) in accordance with the manufacturer’s recommendations.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Smoke 3
|
Data processing |
The data were normalised using quantile normalisation using BioConductor (v.2.12.)'s lumi package in R statistical software
|
|
|
Submission date |
Oct 22, 2013 |
Last update date |
Oct 23, 2013 |
Contact name |
Alexander Peter Sobinoff |
E-mail(s) |
c3045119@uon.edu.au
|
Organization name |
University of Newcastle Australia
|
Department |
Science and IT
|
Lab |
Reproductive Science Group
|
Street address |
University Drive
|
City |
Newcastle |
State/province |
NSW |
ZIP/Postal code |
2308 |
Country |
Australia |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE51530 |
Expression data from adult mouse testis exposed to cigarette smoke in utero |
|