GEO help: Mouse over screen elements for information.
Status
Public on Nov 01, 2013
Title
Th17 cells_Donor 3_Adalimumab
Sample type
RNA
Source name
Th17 cells
Organism
Homo sapiens Characteristics
donor: Donor 3
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using the standard TRIzol protocol (Invitrogen, Carlsbad, CA), but with subsequent RNA clean-up of the water phase using the RNeasy Qiagen MinElute kit (Qiagen, Germantown, MD). The quality of the RNA was confirmed on the Agilent Bioanalyzer using RNA Nano chips (Agilent Technologies, Santa Clara, CA).
Label
biotin
Label protocol
Affymetrix in vitro transcription
Hybridization protocol
Affymetrix EukGE-WS2v5
Scan protocol
Affymetrix cel analysis
Data processing
Bioconductor/R RMA
Submission date
Oct 22, 2013
Last update date
Sep 01, 2016
Contact name
Klaus Stensgaard Frederiksen
Organization name
Novo Nordisk A/S
Department
Molecular Genetics
Street address
Novo Nordisk Park F9.1.07
City
Måløv
ZIP/Postal code
DK-2760
Country
Denmark
Platform ID
GPL16268 Series (1)
GSE51540 Effects of TNF-alpha blocking in sorted Th17 cells from co-cultures of human CD4-positive and CD14-positive cells
Relations Reanalyzed by
GSE86362
Data table header descriptions ID_REF VALUE Log2 RMA signal
Data table ID_REF VALUE AFFX-BioB-3_at
6.527436147
AFFX-BioB-5_at
6.438800258
AFFX-BioB-M_at
7.051438499
AFFX-BioC-3_at
10.48640686
AFFX-BioC-5_at
10.18981616
AFFX-BioDn-3_at
12.40065719
AFFX-BioDn-5_at
11.95081686
AFFX-CreX-3_at
13.93653811
AFFX-CreX-5_at
13.72161452
AFFX-DapX-3_at
12.29304488
AFFX-DapX-5_at
9.692817661
AFFX-DapX-M_at
11.57393617
AFFX-HSAC07/X00351_3_at
13.65838688
AFFX-HSAC07/X00351_5_at
11.88394383
AFFX-HSAC07/X00351_M_at
12.99325092
AFFX-HUMGAPDH/M33197_3_at
13.58113633
AFFX-HUMGAPDH/M33197_5_at
12.96176898
AFFX-HUMGAPDH/M33197_M_at
13.3181029
AFFX-HUMISGF3A/M97935_3_at
9.424315054
AFFX-HUMISGF3A/M97935_5_at
5.104251859
19598 591 Kbytes .Supplementary file Size Download File type/resource GSM1247618_1304_HG-U133_Plus_2_.CEL.gz
4.4 Mb
(ftp) (http) CEL
Processed data included within Sample table
