|
| Status |
Public on Nov 03, 2014 |
| Title |
C.albicans infected zebrafish_15dpi+12hr_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C.albicans infected zebrafish, 15dpi+12hr, repeat2
|
| Organism |
Danio rerio |
| Characteristics |
tissue: whole fish
gender: male
age: 9 months
infection: C.albicans
time: 36 hours post second injection
|
| Treatment protocol |
Each fish was intraperitoneally injected with 1×108 C. albicans cells suspended in 10μL sterile 1× phosphate-buffered saline (PBS)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNeasy Mini Kit (Qiagen, Germany) following the manufacturer's recommendations. RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology, USA) and qualitated by Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 nano labchip kit (Agilent Technologies, USA).
|
| Label |
Cy3
|
| Label protocol |
1.65 μg of Cy3 cRNA for zebrafish array was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 minutes.
|
| |
|
| Hybridization protocol |
The fragmented labeled cRNA was then hybridized to oligo microarray at 60°C for 17 h.
|
| Scan protocol |
After washing and drying by nitrogen gun blowing, microarrays are scanned with an Agilent microarray scanner (Agilent Technologies, USA) at 535 nm for Cy3.
|
| Description |
Gene expression after 15dpi+12hr in C. albicans infected zebrafish
H+P 15dpi+12hr
|
| Data processing |
Scanned images are analyzed by Feature extraction 9.5.3 software (Agilent Technologies, USA), image analysis and normalization software is used to quantify signal and background intensity for each feature.
|
| |
|
| Submission date |
Oct 23, 2013 |
| Last update date |
Nov 03, 2014 |
| Contact name |
Yung-Jen Chuang |
| E-mail(s) |
yjchuang@life.nthu.edu.tw
|
| Phone |
886-3-574-2764
|
| Organization name |
National Tsing Hua University
|
| Department |
Department of Medical Science
|
| Lab |
YJ Chuang
|
| Street address |
No. 101, Sec. 2, Kuang Fu Road
|
| City |
Hsinchu |
| State/province |
Taiwan |
| ZIP/Postal code |
30013 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE51603 |
Innate and adaptive immunity in zebrafish: time course (zebrafish) |
|
