|
| Status |
Public on Jan 01, 2016 |
| Title |
SW620-LATS2N |
| Sample type |
RNA |
| |
|
| Source name |
LATS2N(1-625) overexpression
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SW620
cell type: colorectal cancer
overexpression: LATS2N(1-625)
|
| Growth protocol |
Cells were grown in humidified 5% CO2 incubator in DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit (Qiagen) extraction of total RNA was performed according to the manufacture's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA ( Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix human Genome 133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Gene expression data in SW620 infected with LATS2N(1-625) virus
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings
|
| |
|
| Submission date |
Oct 25, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Xiangming Ding |
| Organization name |
UCLA
|
| Street address |
650 Charles E Young Dr S, Los Angeles, CA 90095
|
| City |
Los angeles |
| State/province |
CA |
| ZIP/Postal code |
91436 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE51715 |
LATS2 regulated genes in human colon cancer cell line |
|
