|
Status |
Public on Apr 22, 2014 |
Title |
PDX_JH011 |
Sample type |
RNA |
|
|
Source name |
Patient derived xenografts from pancreatic tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: Human pancreatic tumor xenografted in six-week-old female athymic nude mice
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA was performed by using Qiagen mini- RNA kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, cRNA was hybridized to an Affymetrix Human Genome U133 Plus 2.0 Array.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from patient derived xenografts from pancreatic tumor
|
Data processing |
The data were analyzed with frozenRMA (fRMA) using rma and quantile as background correction and normalization methods.
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|
|
Submission date |
Oct 28, 2013 |
Last update date |
Apr 23, 2014 |
Contact name |
Manuel Hidalgo |
E-mail(s) |
mhidalgo@cnio.es
|
Phone |
+34917328000
|
Organization name |
CNIO
|
Department |
Clinical Research Programme
|
Lab |
Gastrointestinal Cancer Clinical Research Unit
|
Street address |
C/Melchor Fernandez Almagro 3
|
City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28029 |
Country |
Spain |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE51798 |
Transcriptional dissection of pancreatic tumors engrafted in mice |
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