|
| Status |
Public on Apr 22, 2014 |
| Title |
PDX_JH027 |
| Sample type |
RNA |
| |
|
| Source name |
Patient derived xenografts from pancreatic tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human pancreatic tumor xenografted in six-week-old female athymic nude mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed by using Qiagen mini- RNA kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to an Affymetrix Human Genome U133 Plus 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from patient derived xenografts from pancreatic tumor
|
| Data processing |
The data were analyzed with frozenRMA (fRMA) using rma and quantile as background correction and normalization methods.
|
| |
|
| Submission date |
Oct 28, 2013 |
| Last update date |
Apr 23, 2014 |
| Contact name |
Manuel Hidalgo |
| E-mail(s) |
mhidalgo@cnio.es
|
| Phone |
+34917328000
|
| Organization name |
CNIO
|
| Department |
Clinical Research Programme
|
| Lab |
Gastrointestinal Cancer Clinical Research Unit
|
| Street address |
C/Melchor Fernandez Almagro 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE51798 |
Transcriptional dissection of pancreatic tumors engrafted in mice |
|
