|
| Status |
Public on Dec 27, 2013 |
| Title |
H3K27ac w4h |
| Sample type |
SRA |
| |
|
| Source name |
CUTLL1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CUTLL1
tumor stage: T lymphoblastic leukemia/lymphoma cell line
cell type: T lymphocyte
treatment: GSI 72 hrs, wash out 4 hrs
antibody: H3K27ac (Abcam ab4729)
|
| Treatment protocol |
CUTLL1 cells were treated with vehile (DMSO) or GSI (compound E) for 3 days, and treated with GSI for 3 days following by washing and 4 hours of additional treatement in the absence of GSI
|
| Growth protocol |
CUTLL1 cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, penicillin and streptomycin
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody.
Libraries were prepared according to New England Biolabs instructions accompanying the DNA Sample Prep Master Mix Set 1 (NEB E6040S).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
multiplex
|
| Data processing |
Sequence reads were aligned to the hg19 genome using bowtie
Genome_build: hg19
Supplementary_files_format_and_content: peak files
|
| |
|
| Submission date |
Oct 28, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Chongzhi Zang |
| Organization name |
University of Virginia
|
| Street address |
P. O. Box 800717
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE51800 |
NOTCH1/RBPJ complexes drive target gene expression through dynamic interactions with super-enhancers |
|
| Relations |
| BioSample |
SAMN02385354 |
| SRA |
SRX369135 |
