|
| Status |
Public on Dec 27, 2013 |
| Title |
Dex 10 nM (ECS195_24) |
| Sample type |
RNA |
| |
|
| Source name |
LREX' cells in charcoal-stripped media
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: prostate cancer
treatment: Dex 10 nM
cell line: LREX'
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested from cells using the RNAeasy (Qiagen) Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX HT12 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Slides were scanned using Illumina iScanner
|
| Data processing |
The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
|
| |
|
| Submission date |
Oct 29, 2013 |
| Last update date |
Dec 27, 2013 |
| Contact name |
Vivek Kumar Arora |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
HOPP
|
| Lab |
Charles Sawyers
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE51871 |
Androgen and Glucocorticoid stimulation of LREX' cells in vitro |
| GSE52169 |
Glucocorticoid Receptor Confers Resistance to Anti-Androgens by Bypassing Androgen Receptor Blockade. |
|
