|
| Status |
Public on Dec 27, 2013 |
| Title |
Res - top 75th percentile of GR expressers (900_35) |
| Sample type |
RNA |
| |
|
| Source name |
LnCaP/AR Mouse Xenograft
|
| Organism |
Homo sapiens |
| Characteristics |
cell_line: LnCaP/AR
tissue: prostate cancer
treatment: Enzalutamide
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested from xenografts with Triazole and then further purified using the RNAeasy (Qiagen) Kit.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for Illumina GX HT12 Array. Slides were washed and stained in the Illumina instrument.
|
| Scan protocol |
Slides were scanned using Illumina iScanner
|
| Description |
900_35
|
| Data processing |
The data were analyzed with GenomeStudio using Illumina default analysis settings. No normalization and background correction are performed first, then quantile normalization and background correction are done.
|
| |
|
| Submission date |
Oct 29, 2013 |
| Last update date |
Dec 27, 2013 |
| Contact name |
Vivek Kumar Arora |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
HOPP
|
| Lab |
Charles Sawyers
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE51873 |
LNCAP/AR and a LnCAP/AR subline (LREX') with acquired resistance to 2nd generation antiandrogens |
| GSE52169 |
Glucocorticoid Receptor Confers Resistance to Anti-Androgens by Bypassing Androgen Receptor Blockade. |
|
