|
| Status |
Public on Dec 16, 2014 |
| Title |
PQ 50 mg/kg BW 1 day PQ high 1d_102 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver 1 day after PQ treatment
|
| Organism |
Mus musculus |
| Characteristics |
treatment: PQ 50 mg/kg BW
time: 1 day
strain: C57BL/6
gender: male
age: 10 weeks
weight: 21-27 gram
tissue: liver
|
| Treatment protocol |
Mice were given a single treatment of vehicle or compound by oral gavage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Upon sacrifice, liver was immediately dissected. Central half of the left lateral lobe was collected in RNA later and kept for a maximum of one week at 4 oC and frozen at -80 oC until use for RNA isolation. RNA was isolated using the RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
According to the protocol provided with the Affymetrix 3'IVT Express Labeling Kit (#901229)
|
| |
|
| Hybridization protocol |
According to the Affymetrix Hybridization, Wash and Stain Kit (#901530)
|
| Scan protocol |
According to Affymetrix protocols
|
| Data processing |
Cell Intensity Files generated for each GeneChip using the Affymetrix GeneChip Command Console (v3.0) software were normalized using the Robust Multichip Average (RMA) algorithm from http://arrayanalysis.org (BiGCaT Maastricht University). RMA normalized data were transformed to Log2 by arrayanalysis.org for further analysis. Additionally, the quality of microarray images was inspected visually (http://arrayanalysis.org, BiGCaT Maastricht University). One sample, PQ med 1d, did not meet the quality criteria and was removed leaving 69 samples.
|
| |
|
| Submission date |
Oct 31, 2013 |
| Last update date |
Dec 16, 2014 |
| Contact name |
Alexa Vitins |
| E-mail(s) |
a.vitins@maastrichtuniversity.nl
|
| Organization name |
RIVM
|
| Street address |
P.O. Box 1
|
| City |
Bilthoven |
| ZIP/Postal code |
3720 BA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17226 |
| Series (1) |
| GSE51969 |
Gene expression profiles of acetaminophen, isoniazid, and paraquat treated C57BL/6 mice |
|
