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Status |
Public on Dec 16, 2014 |
Title |
PQ 50 mg/kg BW 1 day PQ high 1d_102 |
Sample type |
RNA |
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Source name |
Mouse liver 1 day after PQ treatment
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Organism |
Mus musculus |
Characteristics |
treatment: PQ 50 mg/kg BW time: 1 day strain: C57BL/6 gender: male age: 10 weeks weight: 21-27 gram tissue: liver
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Treatment protocol |
Mice were given a single treatment of vehicle or compound by oral gavage
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Extracted molecule |
total RNA |
Extraction protocol |
Upon sacrifice, liver was immediately dissected. Central half of the left lateral lobe was collected in RNA later and kept for a maximum of one week at 4 oC and frozen at -80 oC until use for RNA isolation. RNA was isolated using the RNeasy Mini Kit.
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Label |
biotin
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Label protocol |
According to the protocol provided with the Affymetrix 3'IVT Express Labeling Kit (#901229)
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Hybridization protocol |
According to the Affymetrix Hybridization, Wash and Stain Kit (#901530)
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Scan protocol |
According to Affymetrix protocols
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Data processing |
Cell Intensity Files generated for each GeneChip using the Affymetrix GeneChip Command Console (v3.0) software were normalized using the Robust Multichip Average (RMA) algorithm from http://arrayanalysis.org (BiGCaT Maastricht University). RMA normalized data were transformed to Log2 by arrayanalysis.org for further analysis. Additionally, the quality of microarray images was inspected visually (http://arrayanalysis.org, BiGCaT Maastricht University). One sample, PQ med 1d, did not meet the quality criteria and was removed leaving 69 samples.
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Submission date |
Oct 31, 2013 |
Last update date |
Dec 16, 2014 |
Contact name |
Alexa Vitins |
E-mail(s) |
a.vitins@maastrichtuniversity.nl
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Organization name |
RIVM
|
Street address |
P.O. Box 1
|
City |
Bilthoven |
ZIP/Postal code |
3720 BA |
Country |
Netherlands |
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|
Platform ID |
GPL17226 |
Series (1) |
GSE51969 |
Gene expression profiles of acetaminophen, isoniazid, and paraquat treated C57BL/6 mice |
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