|
| Status |
Public on Dec 18, 2013 |
| Title |
A3R_d0_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
rounds of in vivo selection: 3
5-azacytidine status: untreated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAgen Hilden). RNA quality was approved using the Agilent RNA 6000 Nano Kit (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
The samples were labeled according to the Affymetrix GeneChip WT Terminal Labeling protocol (Affymetrix UK Ltd., High Wycombe, UK).
|
| |
|
| Hybridization protocol |
Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
|
| Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from highly metastatic A459 cell line
|
| Data processing |
Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console software Build 1.3.1.187.
|
| |
|
| Submission date |
Nov 06, 2013 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Christian Rohde |
| E-mail(s) |
christian.rohde@uni-heidelberg.de
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE44390 |
DNA Methyltransferase inhibition reverses epigenetically embedded phenotypes in lung cancer preferentially affecting Polycomb target genes |
| GSE52143 |
Changes in global gene expression in lung cancer cell lines A549 (A) and HTB56 (H) [Affymetrix microarrays] |
|
| Relations |
| Reanalyzed by |
GSE86357 |
