|
| Status |
Public on Dec 18, 2013 |
| Title |
A3R_d0_rep2 |
| Sample type |
genomic |
| |
|
| Source name |
A549 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
rounds of in vivo selection: 3
5-azacytidine status: untreated
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA extraction
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulfite conversion of genomic DNA was performed using using the EZ DNA Methylation™ Kit (ZymoResearch, Irvine, CA, USA). Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol.
|
| Scan protocol |
Arrays were imaged using HiScan instrument (Illumina Inc.) using standard recommended Illumina scanner setting
|
| Description |
highly aggressive A549 cell line
|
| Data processing |
Data preprocessing and methylation level extraction was performed using the Genome Studio software v2011.1 (Illumina Inc.) including the Methylation module v1.9.0 and the Illumina Genome Viewer module v1.9.0.
|
| |
|
| Submission date |
Nov 06, 2013 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Christian Rohde |
| E-mail(s) |
christian.rohde@uni-heidelberg.de
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8490 |
| Series (2) |
| GSE44390 |
DNA Methyltransferase inhibition reverses epigenetically embedded phenotypes in lung cancer preferentially affecting Polycomb target genes |
| GSE52144 |
Examination of genome-wide methylation changes in lung cancer cell lines A549 (A) and HTB56 (H) [HumanMethylation27 BeadChip experiments] |
|
