|
| Status |
Public on Mar 03, 2014 |
| Title |
Control-1 |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HaCaT
transfection: siCtrl
tissue: Skin
|
| Treatment protocol |
siRNAs (negative control oligos, siRNAs for NF2 and LATS2, siRNA for p72, siRNAs for DROSHA and DGCR8) were transfected in HaCaT human keratinocytes and maintained at high density prior to RNA sampling.
|
| Growth protocol |
HaCaT immortalized human keratinocytes were cultured in DMEM + 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured cells with TRIzol (Ambion) and processed according to the manufacturer’s protocol.
|
| Label |
miRNA-specific probes
|
| Label protocol |
Labeling was performed according to the manufacturer's protocol (nanoString nCounter analysis, nCounter® miRNA Expression Assays).
|
| |
|
| Hybridization protocol |
Hybridization, blocking and washing were performed according to the manufacturer's protocol (nanoString nCounter analysis, nCounter® miRNA Expression Assays).
|
| Scan protocol |
nCounter Analysis system (nanoString) was used to analyze fluorophore signals.
|
| Data processing |
The miRNA expression in each condition was normalized to positive control probes and all miRNAs.
|
| |
|
| Submission date |
Nov 07, 2013 |
| Last update date |
Mar 03, 2014 |
| Contact name |
Masaki Mori |
| E-mail(s) |
morim@belle.shiga-med.ac.jp
|
| Phone |
+81-775482327
|
| Organization name |
Shiga University of Medical Science
|
| Department |
Molecular Neuroscience Research Center
|
| Street address |
Tsukinowa-cho, Seta
|
| City |
Otsu |
| State/province |
Shiga |
| ZIP/Postal code |
520-2192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17904 |
| Series (2) |
| GSE52186 |
Global microRNA expression analysis in HaCaT human keratinocytes transfected with siRNA(s) for p72, NF2/LATS2, DROSHA/DGCR8, or negative control |
| GSE52276 |
Hippo signaling regulates Microprocessor and links cell density-dependent miRNA biogenesis to cancer |
|
