|
| Status |
Public on Mar 03, 2014 |
| Title |
siNF2/LATS2 + p72-2 |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HaCaT
transfection: siNF2/LATS2 + p72-1
tissue: Skin
|
| Treatment protocol |
p72 expression plasmid or control EGFP plasmid, in addition to siRNAs for NF2 and LATS2, were transfected in HaCaT human keratinocytes and maintained at high density prior to RNA sampling.
|
| Growth protocol |
HaCaT immortalized human keratinocytes were cultured in DMEM + 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cultured cells with TRIzol (Ambion) and processed according to the manufacturer’s protocol.
|
| Label |
miRNA-specific probes
|
| Label protocol |
Labeling was performed according to the manufacturer's protocol (nanoString nCounter analysis, nCounter® miRNA Expression Assays).
|
| |
|
| Hybridization protocol |
Hybridization, blocking and washing were performed according to the manufacturer's protocol (nanoString nCounter analysis, nCounter® miRNA Expression Assays).
|
| Scan protocol |
nCounter Analysis system (nanoString) was used to analyze fluorophore signals.
|
| Data processing |
The miRNA expression in each condition was normalized to positive control probes and all miRNAs.
|
| |
|
| Submission date |
Nov 07, 2013 |
| Last update date |
Mar 03, 2014 |
| Contact name |
Masaki Mori |
| E-mail(s) |
morim@belle.shiga-med.ac.jp
|
| Phone |
+81-775482327
|
| Organization name |
Shiga University of Medical Science
|
| Department |
Molecular Neuroscience Research Center
|
| Street address |
Tsukinowa-cho, Seta
|
| City |
Otsu |
| State/province |
Shiga |
| ZIP/Postal code |
520-2192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17904 |
| Series (2) |
| GSE52205 |
Global microRNA expression analysis in HaCaT human keratinocytes transfected with p72 or control EGFP in addition to siRNAs for NF2 and LATS2. |
| GSE52276 |
Hippo signaling regulates Microprocessor and links cell density-dependent miRNA biogenesis to cancer |
|
