|
| Status |
Public on Jul 31, 2008 |
| Title |
mammary gland-2ug estrogen intraperitoneally-biologic replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
mammary gland
|
| Organism |
Mus musculus |
| Characteristics |
eight week old virgin BALB/cJ mice were ovariectomized followed by a period of one week before treatment to clear endogenous hormones
lymph node-free mammary gland
|
| Treatment protocol |
ovariectomized animals were injected daily with 2ug 17-Beta-estrogen for 4 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ultraspec for RNA extraction (Biotexc, Houston, TX)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U74A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500.
|
| Description |
Mammary gland mRNA expression data from mice treated with estrogen.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150.
|
| |
|
| Submission date |
Aug 09, 2006 |
| Last update date |
Dec 27, 2007 |
| Contact name |
D. Joseph Jerry |
| E-mail(s) |
jjerry@vasci.umass.edu
|
| Phone |
413-545-5335
|
| Fax |
413-545-6326
|
| URL |
http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
|
| Organization name |
University of Massachusetts
|
| Department |
Veterinary & Animal Scineces
|
| Street address |
161 Holdsworth Way
|
| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01003 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5483 |
Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland |
|
