|
| Status |
Public on Nov 19, 2013 |
| Title |
377512 |
| Sample type |
RNA |
| |
|
| Source name |
trisomy 8 t-AML
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone marrow
cell type: trisomy 8 t-AML
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol according to manufacture's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2008, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2/Mouse 430 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 7G06
|
| Description |
Functional Studies in AML and MDS
|
| Data processing |
The data were analyzed with Affymetrix GeneChip Command Console using Affymetrix default analysis settings for MAS5 algorithm and a target intensity of 1500 was used for global scaling as normalization method
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Nov 19, 2013 |
| Contact name |
Matt Walter |
| E-mail(s) |
mjwalter@dom.wustl.edu
|
| Organization name |
washington university in st.louis school of medicine
|
| Department |
oncology
|
| Street address |
4940 parkview
|
| City |
st.louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE52478 |
The DNA Double-Strand Break Response Is Abnormal in Myeloblasts From Patients With Therapy-Related Acute Myeloid Leukemia [Affymetrix] |
| GSE53251 |
The DNA Double-Strand Break Response Is Abnormal in Myeloblasts From Patients With Therapy-Related Acute Myeloid Leukemia |
|
