|
| Status |
Public on Dec 04, 2014 |
| Title |
Lung tissue from cigarette smoke-treated mice at 4 months of age, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N
gender: female
tissue: lung
treatment: cigarette smoke-treated
age: 4 m
|
| Treatment protocol |
Exposure to cigarette smoke as described in the paper
|
| Growth protocol |
Animals housed under spf conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
Biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Gene expression data from lung tissue from cigarette smoke-treated mice at 4 months of age, biological replicate 1
|
| Data processing |
Data including background subtraction (GenomeStudioV2011.1 software, gene expression module version 1.9.0), removal of negative values by introducing an offset, quantile normalisation
|
| |
|
| Submission date |
Nov 19, 2013 |
| Last update date |
Dec 04, 2014 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE52509 |
Cigarette smoke-induced iBALT mediates macrophage activation in a B cell-dependent manner in COPD |
|
