|
Status |
Public on Mar 01, 2007 |
Title |
ICC3996 SA-treated 03 hpt |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Control
|
Organism |
Cicer arietinum |
Characteristics |
Genotype: ICC3996 Age: 14 days (seedling) Tissue: Shoot (stem and leaf)
|
Treatment protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Growth protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Extracted molecule |
total RNA |
Extraction protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Label |
Cy3/Cy5
|
Label protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
|
|
Channel 2 |
Source name |
Treatment
|
Organism |
Cicer arietinum |
Characteristics |
Genotype: ICC3996 Age: 14 days (seedling) Tissue: Shoot (stem and leaf)
|
Treatment protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Growth protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Extracted molecule |
total RNA |
Extraction protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Label |
Cy3/Cy5
|
Label protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
|
|
|
Hybridization protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Scan protocol |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
Description |
The data submitted represents the mean data from three biological replications that inlcuded dye swaps between control and treatment samples
|
Data processing |
See Coram, TE. and Pang, ECK. 2006. Expression profiling of Chickpea genes differentially regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid to reveal pathways of defence-related gene regulation. Plant Biotechnology Journal. Submitted
|
|
|
Submission date |
Aug 11, 2006 |
Last update date |
Mar 01, 2007 |
Contact name |
Tristan Coram |
E-mail(s) |
tristan.coram@ars.usda.gov
|
Organization name |
USDA-ARS, NC State University
|
Street address |
4118 Williams Hall, NC State University
|
City |
Raleigh |
State/province |
NC |
ZIP/Postal code |
27695 |
Country |
USA |
|
|
Platform ID |
GPL4079 |
Series (1) |
GSE5512 |
Chickpea genes regulated by salicylic acid, methyl jasmonate, and aminocyclopropane carboxylic acid |
|
Data table header descriptions |
ID_REF |
|
VALUE |
Log (base 2) transformed mean expression ratio of treatment/control |
FLAG |
Quality control where 0 indicates a good spot and 1 is a bad spot |
ICC3996 SA 03hpi pairMean ratio |
Non-transformed mean expression ratio |
ICC3996 SA 03hpi pairBG Mean ratio |
Non-transformed mean background ratio |
ICC3996 SA 03hpi pairMedian ratio |
Non-transformed median expression ratio |
ICC3996 SA 03hpi pairBG Median ratio |
Non-transformed median background ratio |
ICC3996 SA 03hpi (control)Mean |
Non-transformed control channel mean |
ICC3996 SA 03hpi (control)BG Mean |
Non-transformed control channel background mean |
ICC3996 SA 03hpi (control)Median |
Non-transformed control channel median |
ICC3996 SA 03hpi (control)BG Median |
Non-transformed control channel background median |
ICC3996 SA 03hpiMean |
Non-transformed treatment channel mean |
ICC3996 SA 03hpiBG Mean |
Non-transformed treatment channel background mean |
ICC3996 SA 03hpiMedian |
Non-transformed treatment channel median |
ICC3996 SA 03hpiBG Median |
Non-transformed treatment channel background median |
ICC3996 SA 03hpi pairMean ratio cv |
Non-transformed mean expression ratio coefficient of variation |
ICC3996 SA 03hpi pairBG Mean ratio cv |
Non-transformed mean background ratio coefficient of variation |
ICC3996 SA 03hpi pairMedian ratio cv |
Non-transformed median expression ratio coefficient of variation |
ICC3996 SA 03hpi pairBG Median ratio cv |
Non-transformed median background ratio coefficient of variation |
ICC3996 SA 03hpi (control)Mean cv |
Non-transformed control channel mean coefficient of variation |
ICC3996 SA 03hpi (control)BG Mean cv |
Non-transformed control channel background mean coefficient of variation |
ICC3996 SA 03hpi (control)Median cv |
Non-transformed control channel median coefficient of variation |
ICC3996 SA 03hpi (control)BG Median cv |
Non-transformed control channel background median coefficient of variation |
ICC3996 SA 03hpiMean cv |
Non-transformed treatment channel mean coefficient of variation |
ICC3996 SA 03hpiBG Mean cv |
Non-transformed treatment channel background mean coefficient of variation |
ICC3996 SA 03hpiMedian cv |
Non-transformed treatment channel median coefficient of variation |
ICC3996 SA 03hpiBG Median cv |
Non-transformed treatment channel background median coefficient of variation |