Two-week-old pepper seedlings were drenched with 10 mM BTH or water as a control. Under the same condition, whifefly and whitefly + BTH were treated. Each plant was incubated in a transparent plastic cylinder with a diameter of 15 cm and a height of 50 cm.
Growth protocol
Pepper (Capsicum annuum L. cv. Bukwang) seeds were sterilized with 6% sodium hypochlorite and incubated at 25-28C on MS medium until germination. After germination, pepper plants were transplanted to natural pepper field. soil and were grown at 25-28C with a 12h light/dark period.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted as followed by TRI reagent and treated with 1 U of RNase-free Dnase (Promega, Madison, WI, USA) for 10 min at 37 C. RNA quality and concentration was determined by analysis with an Nanodrop, ND-1000 V3.8.1 bioanalyzer at KRIBB (Daejeon, South Korea).
Label
Cy3
Label protocol
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Hybridization protocol
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
Scan protocol
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
Description
BTH + whitefly treated pepper root
Data processing
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
Understanding cross-communication between aboveground and below ground plant via transcriptome analysis of a sucking insect whitefly-infested pepper plants
Data table header descriptions
ID_REF
VALUE
RMA-normalized, averaged gene-level signal intensity
