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Sample GSM1274197 Query DataSets for GSM1274197
Status Public on Nov 25, 2013
Title mES wild type rep1
Sample type RNA
 
Source name mES wild type
Organism Mus musculus
Characteristics cell type: mES
genotype/variation: wild type
Treatment protocol Cells were untreated or allowed to differentiate by removal of LIFand suspension in hanging drops, encouraging embryoid formation
Growth protocol Pluripotent conditions; cells were grown on a mitotically inactivated MEF line in the presence of LIF
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using Qiagen Rneasy kit, DNA was removed on-column using Rnase free Dnase (Qiagen)
Label Cy3
Label protocol Sample was labelled with cy3 random nonamers
 
Hybridization protocol Array hyb was performed according to Nimblegen protocol
Scan protocol Scanning was done with Genepix 4000B microarry scanner following instruction provided by Nimblegen array protocal
Description Wild type mouse ES cells
Data processing After background correction using theNimbleScan software package, raw data were normalized by RMA (Robust Multi-Array Analysis) method using Bioconductor tool
 
Submission date Nov 24, 2013
Last update date Nov 25, 2013
Contact name Bum-Kyu Lee
E-mail(s) bklee92@gmail.com
Phone 512-232-8046
Organization name University of Texas at Austin
Street address 2506 Speedway Stop A5000
City Austin
State/province TX
ZIP/Postal code 78712
Country USA
 
Platform ID GPL15887
Series (1)
GSE52692 Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

Data table header descriptions
ID_REF
VALUE RMA-normalized, averaged gene-level signal intensity (log2)

Data table
ID_REF VALUE
AB000096 10.46767925
AB000490 7.475403136
AB001425 9.973573635
AB001435 8.80647464
AB001539 13.8483725
AB001750 13.18684688
AB001926 13.03995042
AB003502 13.9576441
AB004048 13.57696714
AB005662 13.56553399
AB005665 5.980387654
AB005909 5.763873571
AB006034 6.278633649
AB006103 5.513216281
AB007407 6.802859027
AB008928 8.528497264
AB009369 6.581691934
AB010088 5.494331398
AB010122 12.86440801
AB011499 13.79174563

Total number of rows: 44170

Table truncated, full table size 918 Kbytes.




Supplementary file Size Download File type/resource
GSM1274197_467447A01_532.pair.gz 2.3 Mb (ftp)(http) PAIR
Processed data included within Sample table

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