|
Status |
Public on Nov 25, 2013 |
Title |
mES wild type rep1 |
Sample type |
RNA |
|
|
Source name |
mES wild type
|
Organism |
Mus musculus |
Characteristics |
cell type: mES genotype/variation: wild type
|
Treatment protocol |
Cells were untreated or allowed to differentiate by removal of LIFand suspension in hanging drops, encouraging embryoid formation
|
Growth protocol |
Pluripotent conditions; cells were grown on a mitotically inactivated MEF line in the presence of LIF
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was harvested using Qiagen Rneasy kit, DNA was removed on-column using Rnase free Dnase (Qiagen)
|
Label |
Cy3
|
Label protocol |
Sample was labelled with cy3 random nonamers
|
|
|
Hybridization protocol |
Array hyb was performed according to Nimblegen protocol
|
Scan protocol |
Scanning was done with Genepix 4000B microarry scanner following instruction provided by Nimblegen array protocal
|
Description |
Wild type mouse ES cells
|
Data processing |
After background correction using theNimbleScan software package, raw data were normalized by RMA (Robust Multi-Array Analysis) method using Bioconductor tool
|
|
|
Submission date |
Nov 24, 2013 |
Last update date |
Nov 25, 2013 |
Contact name |
Bum-Kyu Lee |
E-mail(s) |
bklee92@gmail.com
|
Phone |
512-232-8046
|
Organization name |
University of Texas at Austin
|
Street address |
2506 Speedway Stop A5000
|
City |
Austin |
State/province |
TX |
ZIP/Postal code |
78712 |
Country |
USA |
|
|
Platform ID |
GPL15887 |
Series (1) |
GSE52692 |
Loss of Bright/Arid3A reprograms MEFs to a pluripotent state |
|