|
| Status |
Public on Jan 08, 2014 |
| Title |
Caulobacter_crescentus_wild_type_rep2 (Plac::CCNA_00382) |
| Sample type |
RNA |
| |
|
| Source name |
Caulobacter crescentus, wild type
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype: wild type
|
| Growth protocol |
Cells were cultivated at 28°C in minimal medium (M2G) and harvested in exponential phase (O.D.660nm 0.3).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and the PureLink RNA kit (Invitrogen); a Dnase I treatment was performed before a second purification on PureLink columns. RNA quality and concentration was determined by analysis with a Bioanalyzer and a Nanodrop.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed following the standard operating protocol of Nimblegen. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed following the standard operating protocol of Nimblegen. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed on an Agilent High-Resolution Microarray scanner at 2 µm resolution.
|
| Description |
Caulobacter crescentus, wild type, replicate 2
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Nov 25, 2013 |
| Last update date |
Jan 09, 2014 |
| Contact name |
Diego Gonzalez |
| Organization name |
University of Lausanne
|
| Department |
Department of Fundamental Microbiology
|
| Lab |
Prof. Justine Collier
|
| Street address |
Quartier UNIL-Sorge, Bâtiment Biophore
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17931 |
| Series (1) |
| GSE52722 |
Expression analysis of Caulobacter crescentus NA1000 Plac::CCNA_00382 (ccrM) vs Caulobacter crescentus |
|
