|
Status |
Public on Nov 01, 2006 |
Title |
W7+La(W7-Ca) Vs. DMSO+La(control) - replica 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Arabidopsis seedlings treated with DMSO as control, pre treated with La3+
|
Organism |
Arabidopsis thaliana |
Characteristics |
Twelve-day old Arabidopsis seedlings pretreated with 0.35M LaCl3 for 10 minutes were treated with 0.1% DMSO (control) for 1 hour.
|
Extracted molecule |
total RNA |
Label |
Cy5
|
|
|
Channel 2 |
Source name |
Arabidopsis seedlings treated with W7, pretreated with La3+ (W7-Ca)
|
Organism |
Arabidopsis thaliana |
Characteristics |
Twelve-day old Arabidopsis seedlings pretreated with 0.35M LaCl3 for 10 minutes were treated with 0.6 mM W7 (dissolved in 0.1% DMSO) for 1 hour.
|
Extracted molecule |
total RNA |
Label |
Cy3
|
|
|
|
Description |
Total RNA was extracted from seedling treated for one hour as decribed in the channels Characteristics. Hybridization was performed on an array from Arabidopsis Keck Resource Lab Array (Yale University) containing 12K Arabidopsis ESTs. Data in this platform contains 3 repetitions of 3 independent biological experiments.
|
Data processing |
Per spot and per chip: intensity dependent (lowess)normalization Genespring version GX 7.3.1
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Submission date |
Aug 13, 2006 |
Last update date |
Nov 01, 2006 |
Contact name |
Robert Fluhr |
E-mail(s) |
robert.fluhr@weizmann.ac.il
|
Phone |
972-8-9342175
|
Organization name |
Weizmann Institute of Science
|
Department |
Plant Sciences
|
Street address |
|
City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL242 |
Series (1) |
GSE5517 |
Calcium responsiveness in Arabidopsis using calmodulin antagonists |
|