|
| Status |
Public on Jul 01, 2014 |
| Title |
cell line L-428, knock-down of DUSP5 |
| Sample type |
RNA |
| |
|
| Source name |
Hodgkin’s lymphoma cell line L-428, transfected with vector pcDNA6.2-GW/EmGFP-miR-DUSP5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Hodgkin’s lymphoma cell line
cell line: L-428
transfection: vector pcDNA6.2-GW/EmGFP-miR-DUSP5
|
| Treatment protocol |
Cells were transfected with empty control vector pcDNA6.2-GW/EmGFP-miR-neg or with vector pcDNA6.2-GW/EmGFP-miR-DUSP5
|
| Growth protocol |
Established Hodgkin's lymphoma cells were grown under standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on HG_U133A Microarrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000
|
| Description |
Gene expression data from established Hodgkin's lymphoma cell line after knock-down of DUSP5
|
| Data processing |
The data were analyzed using the MAS5.0 algorithm and Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Nov 29, 2013 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Martin Sebastian Staege |
| E-mail(s) |
martin.staege@medizin.uni-halle.de
|
| Phone |
+49-345-5577280
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Universitätsklinik u. Poliklinik für Kinder- u. Jugendmedizin
|
| Lab |
Children's Cancer Reserach Center
|
| Street address |
Ernst-Grube-Str. 40
|
| City |
Halle (Saale) |
| State/province |
Sachsen-Anhalt |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE52831 |
Gene expression profile of Hodgkin’s lymphoma cells after knock-down of DUSP5 |
|
