|
| Status |
Public on Dec 03, 2013 |
| Title |
Cultured sporozoites 2 |
| Sample type |
SRA |
| |
|
| Source name |
Mosquito salivary glands
|
| Organism |
Plasmodium falciparum NF54 |
| Characteristics |
strain: NF54
developmental stage: sporozoites grown axenically for 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and depleted of rRNA (Ribo-zero; Epicentre)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava1.8.2 software used for basecalling.
Sequenced reads were mapped to Plasmodium falciparum Pf3D7 whole genome using CLC Genomics Workbench with default parameters.
RPKM values were calculated with the CLC Genomics Workbench expression experiments.
Supplementary_files_format_and_content: Files with RPKM values for each Sample.
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ewen F Kirkness |
| E-mail(s) |
ekirknes@jcvi.org
|
| Organization name |
JCVI
|
| Street address |
9704 Medical Center Drive
|
| City |
Rockville |
| State/province |
Maryland |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL18008 |
| Series (1) |
| GSE52867 |
Plasmodium falciparum NF54 Transcriptome |
|
| Relations |
| BioSample |
SAMN02428727 |
| SRA |
SRX385643 |
