|
| Status |
Public on Mar 19, 2014 |
| Title |
male water (5665175044_F) |
| Sample type |
RNA |
| |
|
| Source name |
hypothalamus
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
age: PND 7
Sex: male
treatment: water
tissue: hypothalamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from PND 7 rat pup hypothalami (N = 6/treatment/sex) was isolated using RNeasy Plus Mini Kit (Qiagen, Inc.) according to manufacturer’s instructions and submitted to the Southern California Genotyping Consortium (SCGC) for microarray processing
|
| Label |
biotin
|
| Label protocol |
Standard Illumina biotinylation procedure
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI software
|
| |
|
| Submission date |
Dec 05, 2013 |
| Last update date |
Mar 19, 2014 |
| Contact name |
Toni R Pak |
| E-mail(s) |
tpak@lumc.edu
|
| Organization name |
Loyola University Stritch School of Medicine
|
| Department |
Cell and Molecular Physiology
|
| Street address |
2160 S. First Ave
|
| City |
Maywood |
| State/province |
IL |
| ZIP/Postal code |
60153 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE53028 |
Parental binge alcohol abuse alters F1 generation hypothalamic gene expression in the absence of direct fetal alcohol exposure |
|
