|
| Status |
Public on Dec 01, 2014 |
| Title |
Femoral_21 primary lesion |
| Sample type |
RNA |
| |
|
| Source name |
Femoral artery atherectomy
|
| Organism |
Homo sapiens |
| Characteristics |
age: 81
gender: male
tissue: primary atherosclerotic lesion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (GeneChip Expression 3'- Amplification for IVT Labeling kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from primary atherosclerotic lesion
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 12, 2013 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Einari Aavik |
| Organization name |
University of Eastern Finland
|
| Department |
A.I. Virtanen Institute
|
| Street address |
Neulaniementie 2
|
| City |
Kuopio |
| ZIP/Postal code |
FIN-70211 |
| Country |
Finland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53274 |
Expression data from human femoral artery atherosclerotic lesions |
|
