|
| Status |
Public on Dec 31, 2014 |
| Title |
22_PRE [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
human adipose tissue, pre surgery-induced weight loss
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: subcutaneous adipose
gender: female
condition: PRE surgery-induced weight loss
individual: 22
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following Qiagen protocol.
|
| Label |
biotin
|
| Label protocol |
350 ng of total RNA from each sample, isolated from human adipose tissue, was processed, labelled according to the Affymetrix GeneChip® WT Terminal Labeling Kit.
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix miRNA 3.0 array using an Affymetrix GeneChip Hybridization Oven 645.
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from human adipose tissue.
|
| Data processing |
The data were analyzed using the RMA algorithm (R version 3.0.2) and then LIMMA was applied to calculate significant differential expression between samples.
|
| |
|
| Submission date |
Dec 16, 2013 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE53377 |
Adipose transcriptome and microRNA profiles after surgery-induced weight loss [miRNA] |
| GSE53378 |
Adipose transcriptome and microRNA profiles after surgery-induced weight loss |
|
