|
| Status |
Public on Dec 18, 2013 |
| Title |
BDIV female |
| Sample type |
RNA |
| |
|
| Source name |
rat trigeminal nerve
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: BDIV
gender: female
age: 85 days
treatment: untreated
tissue: trigeminal nerve
|
| Treatment protocol |
no treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from pooled trigeminal nerves was isolated using TRIzol Reagent (Invitrogen) according to manufacturer´s instructions. Quality and quantity of RNA was analysed using Peqlab Nanodrop spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared with the Illumina Total Prep RNA amplification kit (Ambion) according to manufacturer´s instructions.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalized by subtracting background and rank invariant normalization using GenomeStudio Software 2010.
|
| |
|
| Submission date |
Dec 17, 2013 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Andrea Kindler-Röhrborn |
| E-mail(s) |
andrea.kindler@uk-essen.de
|
| Organization name |
Department of Pathology
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45147 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE53390 |
Characterization of gene expression in trigeminal nerves of BDIX, BDIV, BDIX.BDIV-Mss4a and BDIX.BDIV-Mss4d rats of both sexes. |
|
