|
| Status |
Public on Dec 18, 2013 |
| Title |
SRSF1-control-2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 lung cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: lipofectamine alone
|
| Treatment protocol |
Cells were transfected with a 30 nM solution of a non-targeting scramble siRNA (5´-ACGACACGCAGGTCGTCATtt-3´) or an SRSF1 siRNA (5´-TGAAGCAGGTGATGTATGTtt-3´) using lipofectamine
|
| Growth protocol |
Cells were grown in RPMI supplemented with 2 mM glutamine, 10% Fetalclone, 100 U/ml of penicillin and 100 µg/ml of streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNA/DNA Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Labeling was performed by the Proteomics, Genomics and Bioinformatics Core Facility of the Center for Applied Medical Research (CIMA) following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed by the Proteomics, Genomics and Bioinformatics Core Facility of the Center for Applied Medical Research (CIMA) following manufacturer’s instructions.
|
| Scan protocol |
Affymetrix GeneChip 3000 Affymetrix® GeneChip® 3000 GeneChip® 3000
|
| Description |
A549 transfected with lipofectamine alone Experiment 2
|
| Data processing |
Background correction was processed with maximum likelihood estimation (mle). Quantile normalization was performed to adjust the experimental condition of the arrays. Summarized data by probesets.
probe group file: hjay.r1.pgf
meta-probeset file: hjay.r1.mps
|
| |
|
| Submission date |
Dec 17, 2013 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Ravi Datta Sharma |
| E-mail(s) |
ravidattasharma@gmail.com
|
| Organization name |
CEIT
|
| Department |
Biomedical Engineering
|
| Street address |
Parque Tecnológico de San Sebastián, Paseo Mikeletegi, Nº 48,
|
| City |
San Sebastian |
| State/province |
Gipuzkoa |
| ZIP/Postal code |
20009 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18080 |
| Series (1) |
| GSE53410 |
Identification of alternative splicing events regulated by the splicing factor SRSF1 using data from exon-junction microarray technologies |
|
