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Sample GSM1295019

Query DataSets for GSM1295019

Status

Public on Dec 20, 2013

Title

U373 WT + EGF-rep1

Sample type

RNA

Source name

U373 WT + EGF

Organism

Homo sapiens

Characteristics

cell line: U373 WT + EGF
cell type: cell line overexpressing wild type EGFR stimulated EGF for 1hr

Treatment protocol

48 hrs serum starvation plus or minus EGF

Extracted molecule

total RNA

Extraction protocol

Trizol

Label

streptavidin-Alexa647

Label protocol

biotin end-labeled random-octamer oligonucleotide primers

Hybridization protocol

Liu C-G, Calin GA, Volinia S and Croce CM. MicroRNA expression profiling using microarrays. Nature Protocols, 3(4):563-578, 2008

Scan protocol

Liu C-G, Calin GA, Volinia S and Croce CM. MicroRNA expression profiling using microarrays. Nature Protocols, 3(4):563-578, 2008

Data processing

Quantiles normalization was used

Submission date

Dec 19, 2013

Last update date

Dec 20, 2013

Contact name

Frank B Furnari

E-mail(s)

ffurnari@ucsd.edu

Phone

858-534-7819

Organization name

Ludwig Institute for Cancer Research

Lab

Tumor Biology

Street address

9500 Gilman Dr.

City

La Jolla

State/province

ZIP/Postal code

92093

Country

USA

Platform ID

GPL16744

Series (1)

GSE53504

Suppression of MicroRNA-9 by Mutant EGFR Signaling Induces FOXP1 to Enhance Glioblastoma Tumorigenicity

Data table header descriptions
ID_REF
VALUE	Quantile normalized

Data table
ID_REF	VALUE
HSHELA01:CONTROL:Plus/Plus	9.541489628
HSTRNL:CONTROL:Plus/Plus	9.249395849
HUMTRAB:CONTROL:Plus/Plus	8.177608641
HUMTRF:CONTROL:Plus/Plus	9.795411352
HUMTRN:CONTROL:Plus/Plus	8.472308377
HUMTRS:CONTROL:Plus/Plus	9.462106319
HUMTRV1A:CONTROL:Plus/Plus	6.937351662
hsa-let-7a:MATURE:Plus/Plus	10.90817003
hsa-let-7a*:MATURE:Plus/Plus	0
hsa-let-7b:MATURE:Plus/Plus	10.95611369
hsa-let-7b*:MATURE:Plus/Plus	6.820885063
hsa-let-7c:MATURE:Plus/Plus	11.13728789
hsa-let-7d:MATURE:Plus/Plus	10.64161077
hsa-let-7d*:MATURE:Plus/Plus	1.69892712
hsa-let-7e:MATURE:Plus/Plus	9.839280351
hsa-let-7e*:MATURE:Plus/Plus	0
hsa-let-7f:MATURE:Plus/Plus	8.427059886
hsa-let-7f-1*:MATURE:Plus/Plus	0
hsa-let-7f-2*:MATURE:Plus/Plus	0
hsa-let-7g:MATURE:Plus/Plus	8.055117443

Total number of rows: 543

Table truncated, full table size 19 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM1295019_P178_rna88_ucsd_croce_pmt800_81607.gpr.gz	466.6 Kb	(ftp)(http)	GPR
Processed data included within Sample table