|
| Status |
Public on Dec 28, 2013 |
| Title |
Pax3Zic1-injected animal cap, stage 12, induced with Dex, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Pax3Zic1-injected animal cap_stage 12_induced with Dex
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: Xenopus laevis animal cap explant, stage 12
dex treatment: yes
pax3 injection: yes
zic1 injection: yes
chx treatment: no
biological eplicate: 3
|
| Treatment protocol |
Cycloheximide (0,1mg/ml) was then applied to the healed animal caps, from stage 10 to 10.5 (i.e. for 30 min at 23*C), then dexamethasone was added at stage 10.5 to the cycloheximide-containing medium. Explants were rinced and lysed after two additional hours at 23*C, i.e. when sibling embryos reached stage 11.5-12.
|
| Growth protocol |
Xenopus laevis 2-4 cells stage embryos were injected with inducible Pax3-GR+/-Zic1-GR constructs and then grown at 15°C in 1/3 MMR until stage 9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the standard trizol protocol and eppendorf phase-lock gel tubes, followed by proteinase K digestion, and Qiagen Rneasy columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Hybridization was completed using the standard affymetrix protocol
|
| Scan protocol |
Scanning was completed using an Affymetrix Genechip scanner 3000 7G
|
| Description |
PZc3; targets_3I.CEL
|
| Data processing |
The data was analysed with R (v.2.14.2) and the Bioconductor Biobase (v.2.14.0) affy library (v.1.32.1 package) with default settings.
|
| |
|
| Submission date |
Dec 27, 2013 |
| Last update date |
Dec 28, 2013 |
| Contact name |
Anne-Helene Monsoro-Burq |
| E-mail(s) |
Anne-Helene.Monsoro-Burq@curie.fr
|
| Organization name |
Institut Curie
|
| Department |
Université Paris Sud
|
| Lab |
U1021 - UMR3347
|
| Street address |
Bat 110
|
| City |
Orsay |
| ZIP/Postal code |
91405 |
| Country |
France |
| |
|
| Platform ID |
GPL10756 |
| Series (2) |
| GSE53677 |
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers [X_laevis_2] |
| GSE53679 |
Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers |
|
