|
| Status |
Public on Mar 27, 2014 |
| Title |
primary tumor sample intermediate OS 3421K |
| Sample type |
RNA |
| |
|
| Source name |
Human Brain, primary Glioblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
survival: intermediate OS
tissue: Human Brain, primary Glioblastoma
|
| Treatment protocol |
Tumor samples were shock-frozen after resection and stored at -80 °C. Only specimens with a histologically estimated tumor cell content of 80% or more were used for molecular analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
DNA and RNA were extracted by ultracentrifugation.
|
| Label |
biotin
|
| Label protocol |
Sample preparation was done with 2.5 μg total-RNA using the One Cycle Target Labelling and Controls kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from primary Glioblastoma
|
| Data processing |
Raw probe intensities were calibrated and summarized into one expression value per probe set using the hook method, and then quantile-normalised. The expression value of each gene was transformed into log10-scale and centered with respect to its mean value averaged over all samples investigated
|
| |
|
| Submission date |
Dec 30, 2013 |
| Last update date |
Mar 28, 2014 |
| Contact name |
Edith Willscher |
| E-mail(s) |
willscher@izbi.uni-leipzig.de
|
| Organization name |
IZBI
|
| Street address |
Haertelstraße 16-18
|
| City |
Leipzig |
| ZIP/Postal code |
04107 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53733 |
Expression data from primary Glioblastoma in adults |
|
