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Sample GSM1299576 Query DataSets for GSM1299576
Status Public on Mar 27, 2014
Title primary tumor sample intermediate OS 1782H
Sample type RNA
 
Source name Human Brain, primary Glioblastoma
Organism Homo sapiens
Characteristics survival: intermediate OS
tissue: Human Brain, primary Glioblastoma
Treatment protocol Tumor samples were shock-frozen after resection and stored at -80 °C. Only specimens with a histologically estimated tumor cell content of 80% or more were used for molecular analyses.
Extracted molecule total RNA
Extraction protocol DNA and RNA were extracted by ultracentrifugation.
Label biotin
Label protocol Sample preparation was done with 2.5 μg total-RNA using the One Cycle Target Labelling and Controls kit (Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from primary Glioblastoma
Data processing Raw probe intensities were calibrated and summarized into one expression value per probe set using the hook method, and then quantile-normalised. The expression value of each gene was transformed into log10-scale and centered with respect to its mean value averaged over all samples investigated
 
Submission date Dec 30, 2013
Last update date Mar 28, 2014
Contact name Edith Willscher
E-mail(s) willscher@izbi.uni-leipzig.de
Organization name IZBI
Street address Haertelstraße 16-18
City Leipzig
ZIP/Postal code 04107
Country Germany
 
Platform ID GPL570
Series (1)
GSE53733 Expression data from primary Glioblastoma in adults

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity in log10 scale

Data table
ID_REF VALUE
1007_s_at 0.167183027
1053_at -0.190076112
117_at -0.526119029
121_at -0.261172124
1255_g_at -0.445719132
1294_at 0.34996729
1316_at -0.387403644
1320_at 0.02228127
1405_i_at -0.28765593
1431_at 0.489358022
1438_at -0.156416803
1487_at 0.181872264
1494_f_at 0.129412536
1552256_a_at 0.078023172
1552257_a_at 0.196996198
1552258_at -0.639502712
1552261_at -0.025149118
1552263_at 0.093063614
1552264_a_at 0.20444201
1552266_at -0.221739562

Total number of rows: 54675

Table truncated, full table size 1239 Kbytes.




Supplementary file Size Download File type/resource
GSM1299576_1782H.CEL.gz 4.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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