NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1299580 Query DataSets for GSM1299580
Status Public on Mar 27, 2014
Title primary tumor sample long-term OS 2085H
Sample type RNA
 
Source name Human Brain, primary Glioblastoma
Organism Homo sapiens
Characteristics survival: long-term OS
tissue: Human Brain, primary Glioblastoma
Treatment protocol Tumor samples were shock-frozen after resection and stored at -80 °C. Only specimens with a histologically estimated tumor cell content of 80% or more were used for molecular analyses.
Extracted molecule total RNA
Extraction protocol DNA and RNA were extracted by ultracentrifugation.
Label biotin
Label protocol Sample preparation was done with 2.5 μg total-RNA using the One Cycle Target Labelling and Controls kit (Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from primary Glioblastoma
Data processing Raw probe intensities were calibrated and summarized into one expression value per probe set using the hook method, and then quantile-normalised. The expression value of each gene was transformed into log10-scale and centered with respect to its mean value averaged over all samples investigated
 
Submission date Dec 30, 2013
Last update date Mar 28, 2014
Contact name Edith Willscher
E-mail(s) willscher@izbi.uni-leipzig.de
Organization name IZBI
Street address Haertelstraße 16-18
City Leipzig
ZIP/Postal code 04107
Country Germany
 
Platform ID GPL570
Series (1)
GSE53733 Expression data from primary Glioblastoma in adults

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity in log10 scale

Data table
ID_REF VALUE
1007_s_at 0.034770192
1053_at -0.162608213
117_at -1.064768993
121_at -0.028130787
1255_g_at -0.096343956
1294_at -0.217500289
1316_at 0.1386873
1320_at 0.082583862
1405_i_at -0.330600369
1431_at -0.037434021
1438_at -0.214460804
1487_at 0.146778039
1494_f_at 0.017106115
1552256_a_at -0.347259541
1552257_a_at 0.088440659
1552258_at -0.680406977
1552261_at -0.085658525
1552263_at -0.301011418
1552264_a_at 0.286731107
1552266_at -0.237701869

Total number of rows: 54675

Table truncated, full table size 1237 Kbytes.




Supplementary file Size Download File type/resource
GSM1299580_2085H.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap