|
| Status |
Public on Dec 01, 2019 |
| Title |
AS-6h |
| Sample type |
RNA |
| |
|
| Source name |
Tetrahymena thermophila SB210 Total mRNA
|
| Organism |
Tetrahymena thermophila SB210 |
| Characteristics |
time: 6h
agent: arsenic
|
| Treatment protocol |
In the experiment, cells were inoculated at a final concentration of about 4000 cells/ml to 300 ml medium containing 30 μM sodium arsenate dodecahydrate, with plain medium as a negative control. Following 2, 6, 12, 24 h of exposure, appropriate amount of cells were harvested.
|
| Growth protocol |
T. thermophila strain SB210 was cultured axenically at 30°C，in SPP medium (Gorovsky et al., 1975), which consists of 2% proteose peptone (Becton, Dickinson and Company, BD), 0.1% yeast extract (Oxoide), 0.2% glucose (Sinopharm Chemical Reagent Co., Ltd.) and 0.003% ferric citrate (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
the total RNA was extracted using an RNeasy Protect Cell Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions
|
| Label |
Cy3
|
| Label protocol |
Sample labeling protocol provided and performed by NimbleGen.
|
| |
|
| Hybridization protocol |
Sample Hybridization protocol provided and performed by NimbleGen.
|
| Scan protocol |
Sample scan protocol provided and performed by NimbleGen.
|
| Data processing |
Array normalization was performed using the quantile normalization method of Bolstad et al. (2003). Normalized expression values for the individual probes were used to obtain the expression values for a given open reading frame (ORF) by using the robust multiarray average (RMA) procedure as previously described by Irizarry et al. (2003).
|
| |
|
| Submission date |
Jan 06, 2014 |
| Last update date |
Dec 01, 2019 |
| Contact name |
Wei Miao |
| E-mail(s) |
miaowei@ihb.ac.cn
|
| Organization name |
Institute of hydrobiology, CAS
|
| Street address |
donghu south road #7
|
| City |
wuhan |
| State/province |
hubei |
| ZIP/Postal code |
430072 |
| Country |
China |
| |
|
| Platform ID |
GPL6759 |
| Series (1) |
| GSE53836 |
Gene expression of Tetrahymena thermophila SB210 under the arsenic exposure |
|
