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Status |
Public on Dec 01, 2019 |
Title |
AS-12h |
Sample type |
RNA |
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Source name |
Tetrahymena thermophila SB210 Total mRNA
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Organism |
Tetrahymena thermophila SB210 |
Characteristics |
time: 12h agent: arsenic
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Treatment protocol |
In the experiment, cells were inoculated at a final concentration of about 4000 cells/ml to 300 ml medium containing 30 μM sodium arsenate dodecahydrate, with plain medium as a negative control. Following 2, 6, 12, 24 h of exposure, appropriate amount of cells were harvested.
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Growth protocol |
T. thermophila strain SB210 was cultured axenically at 30°C,in SPP medium (Gorovsky et al., 1975), which consists of 2% proteose peptone (Becton, Dickinson and Company, BD), 0.1% yeast extract (Oxoide), 0.2% glucose (Sinopharm Chemical Reagent Co., Ltd.) and 0.003% ferric citrate (Sigma).
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Extracted molecule |
total RNA |
Extraction protocol |
the total RNA was extracted using an RNeasy Protect Cell Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions
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Label |
Cy3
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Label protocol |
Sample labeling protocol provided and performed by NimbleGen.
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Hybridization protocol |
Sample Hybridization protocol provided and performed by NimbleGen.
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Scan protocol |
Sample scan protocol provided and performed by NimbleGen.
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Data processing |
Array normalization was performed using the quantile normalization method of Bolstad et al. (2003). Normalized expression values for the individual probes were used to obtain the expression values for a given open reading frame (ORF) by using the robust multiarray average (RMA) procedure as previously described by Irizarry et al. (2003).
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Submission date |
Jan 06, 2014 |
Last update date |
Dec 01, 2019 |
Contact name |
Wei Miao |
E-mail(s) |
miaowei@ihb.ac.cn
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Organization name |
Institute of hydrobiology, CAS
|
Street address |
donghu south road #7
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City |
wuhan |
State/province |
hubei |
ZIP/Postal code |
430072 |
Country |
China |
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Platform ID |
GPL6759 |
Series (1) |
GSE53836 |
Gene expression of Tetrahymena thermophila SB210 under the arsenic exposure |
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