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Status |
Public on Feb 11, 2016 |
Title |
12 haim-rep3 |
Sample type |
RNA |
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|
Source name |
infecting hyphae of F.graminearum for maize stalk
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Organism |
Fusarium graminearum |
Characteristics |
time point (hours after inoculation): 12
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Treatment protocol |
The maize tissue was fixed and paraffin embedded. Then Laser capture microdissection was used for material collection
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Growth protocol |
Fusarium graminearum hyphae infecting maize stalk
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Extracted molecule |
total RNA |
Extraction protocol |
Picopure RNA isolation kit (Acturus) was used for trace RNA extraction
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression data from infecting hyphae for maize stalk
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Data processing |
The data were analyzed with software R, the RMA package
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Submission date |
Jan 07, 2014 |
Last update date |
Feb 11, 2016 |
Contact name |
Weihua Tang |
Organization name |
Shanghai Inst. Plant Phys. & Eco.
|
Lab |
Plant-Fungal Interaction & Plant Reproductive Biology
|
Street address |
300 Fenglin Rd
|
City |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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|
Platform ID |
GPL18136 |
Series (1) |
GSE53854 |
Gene expression of F.graminearum during maize stalk infection |
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