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Sample GSM1302173 Query DataSets for GSM1302173
Status Public on Feb 11, 2016
Title 12 haim-rep3
Sample type RNA
 
Source name infecting hyphae of F.graminearum for maize stalk
Organism Fusarium graminearum
Characteristics time point (hours after inoculation): 12
Treatment protocol The maize tissue was fixed and paraffin embedded. Then Laser capture microdissection was used for material collection
Growth protocol Fusarium graminearum hyphae infecting maize stalk
Extracted molecule total RNA
Extraction protocol Picopure RNA isolation kit (Acturus) was used for trace RNA extraction
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from infecting hyphae for maize stalk
Data processing The data were analyzed with software R, the RMA package
 
Submission date Jan 07, 2014
Last update date Feb 11, 2016
Contact name Weihua Tang
Organization name Shanghai Inst. Plant Phys. & Eco.
Lab Plant-Fungal Interaction & Plant Reproductive Biology
Street address 300 Fenglin Rd
City Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL18136
Series (1)
GSE53854 Gene expression of F.graminearum during maize stalk infection

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1415670_at 2.80897463
1415671_at 2.462159862
1415672_at 2.101197666
1415673_at 2.338651174
1415674_a_at 3.61878659
1415675_at 3.990798525
1415676_a_at 3.345788648
1415677_at 2.500244114
1415678_at 3.54159915
1415679_at 2.740473907
1415680_at 3.226618522
1415681_at 2.829889803
1415682_at 2.316938855
1415683_at 2.665780257
1415684_at 2.995737226
1415685_at 2.565995324
1415686_at 2.401545084
1415687_a_at 2.897103526
1415688_at 2.880438399
1415689_s_at 2.273309798

Total number of rows: 18069

Table truncated, full table size 447 Kbytes.




Supplementary file Size Download File type/resource
GSM1302173_CB2007190-B3.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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